MZT(株)ゼブラフィッシュ創薬研究所

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最近の記事

2024/08/06
Validation of a new protocol for a zebrafish MEFL (malformation or embryo-fetal lethality) test method that conforms to the ICH S5 (R3) guideline.
2024/05/21
In vivo assessment of individual and total proteinuria in zebrafish larvae using the solvatochromic compound ZMB741
2021/10/31
Generation of a Transgenic Zebrafish Line for In Vivo Assessment of Hepatic Apoptosis
2021/08/19
Patient-Derived Cancer Xenograft Zebrafish Model (PDXZ) for Drug Discovery Screening and Personalized Medicine
2021/07/09
Establishment of a Quality Control Protocol for Zebrafish Developmental Toxicity Studies
2020/10/13
Gap junction protein beta 4 plays an important role in cardiac function in humans, rodents, and zebrafish
2020/05/28
A novel orexin antagonist from a natural plant was discovered using zebrafish behavioural analysis
2019/10/15
C3orf70 Is Involved in Neural and Neurobehavioral Development
2019/09/22
Generation of a Triple-Transgenic Zebrafish Line for Assessment of Developmental Neurotoxicity during Neuronal Differentiation
2019/07/17
Aging-associated microstructural deterioration of vertebra in zebrafish

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2015/10/13
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Pharmacological profiling of zebrafish behavior using chemical and genetic classification of sleep-wake modifiers

frontiers
Pharmacological profiling of zebrafish behavior using chemical and genetic classification of sleep-wake modifiers
Yuhei Nishimura, Shiko Okabe, Shota Sasagawa, Soichiro Murakami, Yoshifumi Ashikawa, Mizuki Yuge, Koki Kawaguchi, Reiko Kawase, Toshio Tanaka

Sleep-wake states are impaired in various neurological disorders. Impairment of sleep-wake states can be an early condition that
exacerbates these disorders. Therefore, treating sleep-wake dysfunction may prevent or slow the development of these diseases.
Although many gene products are likely to be involved in the sleep-wake disturbance, hypnotics and psychostimulants clinically used
are limited in terms of their mode of action and are not without side effects. Therefore, there is a growing demand for
developing new hypnotics and psychostimulants with high efficacy and few side effects. Towards this end, animal models are
indispensable for use in genetic and chemical screens to identify sleep-wake modifiers. As a proof-of-concept study, we performed
behavioral profiling of zebrafish treated with chemical and genetic sleep-wake modifiers. We were able to demonstrate that
behavioral profiling of zebrafish treated with hypnotics or psychostimulants from 9 to 10 days post fertilization was sufficient to
identify drugs with specific modes of action. We were also able to identify behavioral endpoints distinguishing GABA-A modulators
and hypocretin (hcrt) receptor antagonists and between sympathomimetic and non-sympathomimetic psychostimulants. This
behavioral profiling can serve to identify genes related to sleep-wake disturbance associated with various neuropsychiatric
diseases and novel therapeutic compounds for insomnia and excessive daytime sleep with fewer adverse side effects.