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| 2012/12/30 |
|
MieKomachiシリーズを、朝日新聞が紹介してくれました。
食行動制御遺伝子と医薬品の探索研究を、教室独自に開発したMieKomachiシリーズを活用することにより、更なるスループット向上が実現し、真にゲノムワイドかつ大規模ケミカルライブラリーに対応できることを、紹介してくれました。今後さらに急速に現在１６種類のMieKomachiシリーズを拡充し、世界におけるゼブラフィッシュによるシステムズ薬理学研究に貢献することが必要となりました。
Research Article
A High-Throughput Fluorescence-Based Assay System for Appetite-Regulating Gene and Drug Screening
Abstract
The increasing number of people suffering from metabolic syndrome and obesity is becoming a serious problem not only in developed countries, but also in developing countries. However, there are few agents currently approved for the treatment of obesity. Those that are available are mainly appetite suppressants and gastrointestinal fat blockers. We have developed a simple and rapid method for the measurement of the feeding volume of Danio rerio (zebrafish). This assay can be used to screen appetite suppressants and enhancers. In this study, zebrafish were fed viable paramecia that were fluorescently-labeled, and feeding volume was measured using a 96-well microplate reader. Gene expression analysis of brain-derived neurotrophic factor (bdnf), knockdown of appetite-regulating genes (neuropeptide Y, preproinsulin, melanocortin 4 receptor, agouti related protein, and cannabinoid receptor 1), and the administration of clinical appetite suppressants (fluoxetine, sibutramine, mazindol, phentermine, and rimonabant) revealed the similarity among mechanisms regulating appetite in zebrafish and mammals. In combination with behavioral analysis, we were able to evaluate adverse effects on locomotor activities from gene knockdown and chemical treatments. In conclusion, we have developed an assay that uses zebrafish, which can be applied to high-throughput screening and target gene discovery for appetite suppressants and enhancers.
Citation: Shimada Y, Hirano M, Nishimura Y, Tanaka T (2012) A High-Throughput Fluorescence-Based Assay System for Appetite-Regulating Gene and Drug Screening. PLoS ONE 7(12): e52549. doi:10.1371/journal.pone.0052549
Editor: Zhiyuan Gong, National University of Singapore, Singapore
Received: August 13, 2012; Accepted: November 20, 2012; Published: December 26, 2012
関連リンク
- A High-Throughput Fluorescence-Based Assay System for Appetite-Regulating Gene and Drug Screening
- PLOS ONE HP
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