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ゼブラフィッシュストレスの非侵襲的解析法

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2017/03/12
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ゼブラフィッシュストレスの非侵襲的解析法

ゼブラフィッシュの飼育管理、病態モデル、薬効評価などにおいて、ストレス反応評価は、基本的解析となる。しかも、非侵襲的方法がある方法があるとしたら、全ての研究者にとって見逃せない方法である。それは、尿中コルチゾールです。この方法は、ヒト臨床において伝統的に活用されてきた尿検査に応用されることになり、我々はそのいくつかを可能にしている。
The effect of common husbandry conditions (crowding, social environment, water quality, handling, and background color) on the cortisol stress response in adult zebrafish, Danio rerio, was investigated to check the usefulness of zebrafish as a model organism in aquaculture research. In addition, a noninvasive methodology for assessing stress was evaluated.
Zebrafish showed a fast cortisol response with high values at 30 min that returned to basal levels within 2 h of poststress.
There was a significant positive correlation between trunk cortisol concentrations and the free water cortisol rate (r2 = 0.829&#8211;0.850, p < 0.001), indicating that measurement of the water-borne cortisol release rate may serve as a noninvasive and reliable stress indicator at the population level. Crowding resulted in 13- to 21-fold greater mean trunk cortisol concentrations compared with controls.
However, even at low stocking density (2&#8211;5 fish/L), the maintenance cost was higher than the one at higher densities (10 fish/L) due to the formation of dominance hierarchies.
The background color affected trunk cortisol concentrations, with fish exposed to brighter backgrounds (green and white) showing 3- to 8-fold greater mean trunk cortisol concentrations than fish exposed to a black background or transparent aquaria. Fish exposed to
high stocking densities for 2 h or 5 days had similar high mean trunk cortisol levels, indicating that exposure of fish for the period of 2 h to a specific stressor may represent a chronic situation in zebrafish.
It is concluded that adult laboratory zebrafish had a preference for a transparent or black background aquarium, at a number of 10
individuals per 2 L of available water volume, to express their normal behavior and avoid increased cortisol stress reaction.

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