ＭＺＴ（株）ゼブラフィッシュ創薬研究所

蛍光in vivoハイスループット摂食量測定技術による定量的システムズ薬理学研究

第86回日本薬理学会年会

Zebrafish-based Quantitative and Systems Pharmacology for Appetite-regulating Gene and Drug Screening.

Yasuhito Shimada, Junya Kuroyanagi, Noriko Umemoto, Beibei Zhang, Yuhei Nishimura and Toshio Tanaka

The increasing number of people suffering from metabolic syndrome and obesity is becoming a serious problem not only in developed countries, but also in developing countries. However, there are few agents currently approved for the treatment of obesity. Those that are available are mainly appetite suppressants and gastrointestinal fat blockers. We have developed a simple and rapid method for the measurement of the feeding volume of zebrafish. This assay can be used to screen appetite suppressants and enhancers. In this study, zebrafish were fed viable paramecia that were fluorescently-labeled, and feeding volume was measured using a 96-well microplate reader. Gene expression analysis of brain-derived neurotrophic factor (bdnf), knockdown of appetite-regulating genes, and the administration of clinical appetite suppressants revealed the similarity among mechanisms regulating appetite in zebrafish and mammals. In combination with behavioral analysis, we were able to evaluate adverse effects on locomotor activities from gene knockdown and chemical treatments. In conclusion, we have developed an assay that uses zebrafish, which can be applied to high-throughput screening and target gene discovery for appetite suppressants and enhancers.

ＭＺＴ（株）ゼブラフィッシュ創薬研究所

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蛍光in vivoハイスループット摂食量測定技術による定量的システムズ薬理学研究