最近の記事

2020/05/26
Zebrafish-Based Precision Oncology
2020/05/25
Zebrafish-Based Phenotype Screening
2020/05/23
phenomicsはzebrafishにより爆発する。
2020/02/21
High-Throughput Cardiotoxicity Screening System for Onco-Cardiology
2020/02/13
zebrafish-based onco-cardiology
2020/01/01
次世代プレシジョンメディシン元年
2019/11/21
次世代ゼブラフィッシュ創薬スクリーニングとプレシジョンメディシン
2019/08/27
患者がん移植ゼブラフィッシュモデル（PDXZ） への展開
2019/07/25
次世代ゼブラフィッシュ創薬へのイノベーション
2019/07/12
次世代プレシジョンメディシンNext Generation Precision Medicine

一覧に戻る

2011/06/09
  • CiteULike
  • reddit
  • StumbleUpon
  • linkedin
  • Delicious
  • Mendeley
  • はてなブックマーク
  • Youtube
  • Google+
  • Twitter
  • Facebook

Microinjection into Zebrafish Embryos

Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.

Commented by Y. Shimada

関連ファイル